Applications
Learn how scientists are using TROVO to design custom experiments and workflows for their research applications.
The complete TROVO workflow for TIL-based immunotherapy: from tumor tissue dissociation through microwell seeding, longitudinal co-culture imaging, functional clone selection, and live cell recovery. Designed for clinical manufacturing contexts where sample scarcity and reproducibility are critical.
Identify and quantify tumor-killing TILs from clinical samples using TROVO’s Z-score-based potency assessment. By comparing each co-culture microwell against 50 matched tumor-only controls, TROVO generates statistically rigorous killer clone metrics from as few as ~10,000 cells — distinguishing responding from non-responding patient samples without predefined markers.
Rank CAR-T and TCR-T constructs by real-time killing kinetics — not surface markers. TROVO tracks cytotoxicity and persistence live over 12 days across thousands of individual co-cultures, separating true persistence clones from short-term killers before any downstream investment. Retrieved clones maintain full phenotypic expression and functionality post-expansion.
TROVO’s per-clone kinetic data can be fit to Lotka-Volterra predator-prey equations to derive a quantitative killing rate constant (k_eff) for each individual clone — separating intrinsic single-cell killing capacity from cooperative “wolf-pack” killing at higher effector densities. In vitro k_eff profiles fitted from 3,000 microcultures (R²>0.95) correlate directly with in vivo tumor control and survival in NSG mouse models.
TROVO is an “all in one” high throughput imaging system capable of monitoring individual B cell IgG secretion over time with the ability to recover cells of interest then. TROVO can print 20k of hydrogel-based microwells on a 6-well plate, allowing microenvironments to be established. B cells can easily be incubated in our microwells with antigen-coated beads or target cells (suspension/adherent) that can be labeled with a secondary antibody to detect secretion within just 2 hours. These sequestered cells can be tracked over time, captured, and recovered for expansion or sequencing.
Optimize T cell clone selection with Enrich TROVO. This platform uses hydrogel microwells and imaging to track T cell-tumor interactions, identifying top-performing clones for CAR-T and TCR-T development. TROVO’s efficient workflow isolates viable T cells for downstream analysis, expansion, and functional validation in just 12 days.
With very little human interaction, TROVO can bin microculture movies into several meaningful function-based groups. Highly promising T cell clones can easily and automatically be distinguished from undesirable clones. TROVO’s unique AI grouping of microculture movies eliminates user variability for cell selection and removes the need to stringently develop cell segmentation strategies, greatly increasing your lab’s efficiency.
Optimize live cell enrichment for single-cell RNA sequencing with Enrich TROVO. Using light-induced microgel lithography and image-based selection, TROVO gently isolates live cells from fresh or frozen tissue samples. Its fluidics-free design prevents clogging, preserves viability, and ensures higher transcriptomic integrity. Process up to three samples in four hours with results that maintain tissue-specific features and enable diverse cell detection in 10X Genomics analyses.
