Bridging the gap between in vitro and in vivo analysis
Developing novel cellular therapeutics requires a deeper understanding of normal in vivo cell behavior over time. Existing high-throughput cell analysis platforms are expensive and incapable of studying cell behavior over extended timelines. In comparison, in vitro cell capture technologies require harsh single-cell dissociation methods and cell sorting that can cause stress and damage to the cells outside of their in vivo cell culture conditions.
TROVO allows cells to be monitored under native culture conditions, enabling longer experimental timelines and cell behavior analysis.
More flexibility in experimental design with biologically meaningful cell culture environments
High-throughput
Analyze up to 24,000 single cells
Culture Longer
Culture up to weeks more than other systems
Microfluidics-free Cell Culture
Keep cells in native culture medium
Controlled Cell Density
Plate cells in physiologically relevant conditions
Phenotypic Selection
Select desired cells based on behavior or phenotype
Know Your Cells
Collect data over longer timelines for each microwell
Enrich for Live Cells
Visually confirm and enrich for viable cells
Minimal Sample Loss
Work with precious samples without losing cells
How does TROVO work?
TROVO works with standard cell culture plates under native cell culture conditions. The open platform provides scientists flexibility to design experimental assays that meet their needs. TROVO delivers two unique modes of cell capture selection: microwell-based capture and free space capture.
Microwell-based capture mode employs creation of micron-sized wells to enable high-throughput, long-term cell culture experiments.
- Cell culture plates with Enrich hydrogel are loaded into TROVO.
- Using a patented, light-based microgel lithography and LCD dynamic mask, TROVO can create up to 24,000 custom microwells of any size and shape in a 6-well culture plate.
- Wells are visually identified and indexed for downstream data collection and analysis.
- Cells are then seeded into the wells, imaged, and allowed to culture for up to weeks. During incubation, additional cells can be added to test functional interactions and phenotypes.
- Each indexed well can be imaged at desired timepoints, including at initial seeding to provide visual confirmation of single-cell clones. Indexed images can be analyzed to provide cell kinetics and more accurate profiling data to identify the best functional phenotype.
- Following visual identification, TROVO can capture and maintain viability and functionality of the desired cells for downstream applications including NGS and single-cell genomics/transcriptomics analysis. Undesired cells are simply aspirated away from the captured cells.
Free space capture mode enables you to capture and select the cells you want using an open selection tool with Enrich hydrogel. With this mode, you have the flexibility to perform positive, negative, and free form selection.
- Positive selection enables capture of desired cells while undesired cells, dead cells, and debris are washed away.
- Negative selection enables capture of undesired cells, dead cells, and debris. Live, desired cells are aspirated and transferred to a new culture dish or harvested for downstream applications.
- Free form selection is a limitless, precise selection tool that enables you to isolate cells or networks of cells within the culture plate.
Eliminate ambiguity and discover the best cells faster
Longer culture time enables greater confidence in data
- Culture and monitor cells for up to weeks longer than other systems
- Statistically and biologically more relevant to gain more confidence in your data
Figure 1. Birdseye view of microwells collected at days 0 and 8 (Green: Live tumor cells, Yellow: T cells, Black: dead cells).
Figure 2. Ratio of growth versus death of effector T-cells to tumor cells in the microwells on days 1, 4, and 8 (Effector:Tumor = 10K:100K). In this experiment, the number of T-cells and cancer cells are increasing over time. Bottom right quadrant illustrates microwells with greater T-cell proliferation and tumor cell death (black, inside blue circle). Top right quadrant demonstrates both tumor and T-cell proliferation over time (light blue, inside purple circle). Whereas the top left quadrant demonstrates an increase T-cell death and tumor cell proliferation (dark blue).
Figure 3. Analysis of microwell co-cultures for at least 8 days achieves more representative results compared to only 4 days. Initial comparison of co-cultures at day 4 suggest wells 2, 3, and 4 are ideal CAR-T cell clones for further development and analysis based on tumor killing rate. Whereas after 8 days of co-culture, CAR-T cell clones in wells 1, 2, and 3 appear to be the best candidates for their ability to kill tumor cells and persist without exhaustion and well 4’s CAR-T cells have exhausted their tumor killing capacity. In the final column, kinetic data presented over 8 days illustrates that CAR-T clones have unique phenotypes for their killing behavior. Longer co-cultures and more accurate profiling reveals this. (Each column represents the data collected on the same day. Green: Live tumor cells, Yellow: T cells, Black: dead cells).
Isolate rare cells without cell loss
- Successfully identify and capture rare cells
- No plate-to-plate transfers ensures no to minimal cell loss
Figure 1. Typical whole well image montage generated from single images. GFP-positive suspension cells were spiked in with non-fluorescently labeled cells. GFP cells were successfully identified (red circles). GFP-positive cells were individually selected for capture.
1. Before Capture
2. After Capture
3. After Wash
4. After Digest
Figure 2. TROVO provides seamless identification and cell capture technology. Captured cells remain adhered to the culture plate while non-adhered cells can be washed away. Following removal of non-adherent cells, captured cells can be visualized on TROVO and then retrieved with a gentle enzymatic solution that dissolves the surrounding gel.
Cell capture screening services
- Are you interested, but need support in discovering your next great cell therapy? Enrich offers in-house cell culture processing of your samples. Our team can process samples for a variety of applications, including:
- High-throughput screening to select the best functional T-cell (CAR-T, TCR-T, TIL) clone for therapeutic candidates
- Find your best in vivo CAR-T in one month
- Have the ultimate confidence in your CAR-T screening data by using a technology and workflow that have been validated to be predictive of in vivo success in previous studies
- Decrease volume of in vivo studies required with in vivo-predictive Enrich Biosystems screening data
- Fast generation of cell lines and organoids
- Bulk cell or tissue sample processing for single-cell omics
Contact us with your project interests and we will respond within 1 business day.
Contact Us
Are you interested in learning more about Enrich Biosystem’s TROVO all-in-one platform? Fill out this form to contact us and a member of our team will reach out to answer your questions or set up a demo.